| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Holdemania filiformis | GGGGATGGTCATTGCCAGT | AGCGCATTCAGCACCAGA | 59.69 | 59.65 | 249 | 94 |
100.00%
|
50.00%
|
| Primer 62 | Holdemania filiformis | TTGCTGGGGTGTGCTTCA | CCTTCACGGTTGCCAAAGC | 59.40 | 60.01 | 266 | 94 |
100.00%
|
50.00%
|
| Primer 63 | Holdemania filiformis | TTGCTGGGGTGTGCTTCA | CAGTCCTTCACGGTTGCCA | 59.40 | 60.23 | 270 | 94 |
100.00%
|
50.00%
|
| Primer 64 | Holdemania filiformis | TTGCTGGGGTGTGCTTCA | AGTCCTTCACGGTTGCCA | 59.40 | 58.76 | 269 | 94 |
100.00%
|
50.00%
|
| Primer 65 | Holdemania filiformis | ACCGATCGAACGACGCTT | TGATCCGCTGCCCTGAAA | 59.44 | 58.92 | 230 | 94 |
100.00%
|
50.00%
|
| Primer 66 | Holdemania filiformis | ACCGATCGAACGACGCTT | GCTGCCCTGAAACGAAAGC | 59.44 | 60.08 | 224 | 94 |
100.00%
|
50.00%
|
| Primer 67 | Holdemania filiformis | ACCGATCGAACGACGCTT | CATGATCCGCTGCCCTGAA | 59.44 | 60.15 | 232 | 94 |
100.00%
|
50.00%
|
| Primer 68 | Holdemania filiformis | ACCGATCGAACGACGCTT | CGCTGCCCTGAAACGAAAG | 59.44 | 59.79 | 225 | 94 |
100.00%
|
50.00%
|
| Primer 69 | Holdemania filiformis | ACCGATCGAACGACGCTT | GCCCTGAAACGAAAGCACC | 59.44 | 59.71 | 221 | 94 |
100.00%
|
50.00%
|
| Primer 70 | Holdemania filiformis | ACCGATCGAACGACGCTT | ATGATCCGCTGCCCTGAA | 59.44 | 58.68 | 231 | 94 |
100.00%
|
50.00%
|